Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.
Additionally, we also offer 2-color beads in the color combinations red/orange, red/green and red/blue.
On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.
Fields of Application:
Advantages:
using the MIP renderer in Huygens Professional
Bead R | |
---|---|
Color | red |
Available fluorophores | ATTO647N |
Alternative fluorophores | Abberior STAR 635P |
Recommended laser source | 630-650 nm |
Diameter | 23 nm |
Surface density | ≈ 1/µm² |
Price (sample on glass slide) | 300,-€ |
Price (sample in solution) | 500,-€ |
Bead O - brightline | Bead Y - brightline | Bead G - brightline | Bead B - brightline | |
---|---|---|---|---|
Color | orange | yellow | green | blue |
Available fluorophores | ATTO 594 | ATTO 565 | ATTO 542 | Alexa Fluor 488 |
Alternative fluorophores | - | - | - | - |
Recommended laser source | 565-610 nm | 540 - 585 nm | 515-540 nm | 480-505 nm |
Diameter | 23 nm | 23 nm | 23 nm | 23 nm |
Surface density | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² |
Price (sample on glass slide) | 350,-€ | 350,-€ | 350,-€ | 350,-€ |
Price (sample in solution) | 550,-€ | 550,-€ | 550,-€ | 550,-€ |
Bead RO - brightline | Bead RG - brightline | Bead RB - brightline | |
---|---|---|---|
Color | red / orange | red / green | red / blue |
Available fluorophores | ATTO647N / ATTO594 | ATTO647N / ATTO542 | ATTO647N / Oregon Green 488 |
Recommended laser source | 630-650 nm, 565-610 nm | 630-650 nm, 515-540 nm | 630-650 nm, 480-505 nm |
Diameter | 23 nm | 23 nm | 23 nm |
Surface density | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² |
Price (sample on glass slide) | 450,-€ | 450,-€ | 450,-€ |
Price (sample in solution) | 650,-€ | 650,-€ | 650,-€ |
The standard version of our GATTA-Beads you can still request on our legacy products page.
Fluorophore | Our notation | Excitation / nm |
Emission / nm |
ATTO 647N | R | 647 |
664 |
Abberior Star 635P | R | 635 |
651 |
ATTO 594 | O | 594 | 626 |
ATTO 565 | Y | 564 | 590 |
ATTO 542 | G | 542 | 562 |
Cy3 | G | 554 | 568 |
Alexa Fluor 555 | G | 555 | 580 |
Oregon Green 488 | B | 488 | 526 |
Alexa Fluor 488 | B | 488 | 525 |
ATTO 490LS | B | 490 | 658 |
Full size super-resolution Image of GATTA-Bead R
Full size super-resolution Image of GATTA-Bead G
Full size super-resolution Image of GATTA-Bead B
Full size confocal Image of GATTA-Beads G
Full size confocal Image of GATTA-Beads G - brightline
Image Courtesy: Christian Jüngst, CECAD Imaging Facility (confocal)
J.J. Schmied, R. Dijkstra, M. Scheible, G. M. R. De Luca, J. J. Sieber, GATTAquant GmbH, Scientific Volume Imaging (SVI), Leica Microsystems (2016): Measuring the 3D STED PSF with a new Type of Fluorescent Beads – Science Lab