GATTA-Beads

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Small, bright and cutting-edge. Experience the highest brightness density in the world.

Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.

Additionally, we also offer 2-color beads in the color combinations red/orange, red/green and red/blue.

On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.

New super-resolution Button GATTA-Beads - brightline

The GATTAquant Brightline series delivers you a much better tool for your experiments and calibration measurements we now developed a new series of GATTA-Beads featuring an even higher fluorescence brightness but without any loss of compactness compared to previous GATTA-Beads.
 
Currently we provide as colors orange (ATTO 594), yellow (ATTO 565), green (ATTO 542) and blue (Alexa Fluor 488 and Oregon Green 488) beads.

Fields of Application:

  • Setup calibration
  • 2D STED measurements
  • 3D STED PSF distillation and deconvolution
  • Tracking and drift-correction


Advantages:

  • Size: d = 23 nm
  • Brightness: the highest brightness density
  • Homogeneity: super small size and intensity distribution
  • Flexibility: change color or add chemical groups

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3D stack of GATTA-Beads R measured at VUmc, Amsterdam, on a Leica TCS SP8 STED 3X. 3D PSF distillation and rendering by Scientific Volume Imaging using the MIP renderer in Huygens Professional

product properties of GATTA-Beads

Bead R
Color red
Available fluorophores ATTO647N
Alternative fluorophores Abberior STAR 635P
Recommended laser source 630-650 nm
Diameter 23 nm
Surface density ≈ 1/µm²
Price (sample on glass slide) 300,-€
Price (sample in solution) 500,-€

New super-resolution Button product properties of GATTA-Beads - brightline

Bead O - brightline Bead Y - brightline Bead G - brightline Bead B - brightline
Color orange yellow green blue
Available fluorophores ATTO 594 ATTO 565 ATTO 542 Alexa Fluor 488
Alternative fluorophores - - - -
Recommended laser source 565-610 nm 540 - 585 nm 515-540 nm 480-505 nm
Diameter 23 nm 23 nm 23 nm 23 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Price (sample on glass slide) 350,-€ 350,-€ 350,-€ 350,-€
Price (sample in solution) 550,-€ 550,-€ 550,-€ 550,-€
Bead RO - brightline Bead RG - brightline Bead RB - brightline
Color red / orange red / green red / blue
Available fluorophores ATTO647N / ATTO594 ATTO647N / ATTO542 ATTO647N / Oregon Green 488
Recommended laser source 630-650 nm, 565-610 nm 630-650 nm, 515-540 nm 630-650 nm, 480-505 nm
Diameter 23 nm 23 nm 23 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Price (sample on glass slide) 450,-€ 450,-€ 450,-€
Price (sample in solution) 650,-€ 650,-€ 650,-€

The standard version of our GATTA-Beads you can still request on our legacy products page.

fluorophore properties

 Fluorophore Our notation Excitation / nm
Emission / nm
ATTO 647N R  647
664
Abberior Star 635P R  635
651
ATTO 594 O 594 626
ATTO 565 Y 564 590
ATTO 542 G 542 562
Cy3 G 554 568
Alexa Fluor 555 G 555 580
Oregon Green 488 B 488 526
Alexa Fluor 488 B 488 525
ATTO 490LS B 490 658

 

 

product sheet
Full size super-resolution Images
Publications

J.J. Schmied, R. Dijkstra, M. Scheible, G. M. R. De Luca, J. J. Sieber, GATTAquant GmbH, Scientific Volume Imaging (SVI), Leica Microsystems (2016): Measuring the 3D STED PSF with a new Type of Fluorescent Beads Science Lab

References / Experience Reports

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Kontakt

GATTAquant GmbH
Staffelseestraße 8
DE-81477 München
Phone: +49 89 2153 720 80
INFO@GATTAQUANT.COM

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