Being the first super-resolution microscopy technique the STED method revolutionized the light microscopy. With the nanorulers from the GATTA-STED series now adequate calibration probes are finally available. GATTA-STED 3D 90R Expert Line nanorulers are single color 3D structures carrying two fluorescent marks out of a dense arrangements of the high quantum yield dye ATTO 647N. The spots are separated by 90 nm and oriented in axial direction.
The 3D nanorulers are intended for use with high-performing STED systems with extraordinary z resolution and are only recommended for very experienced users.
On request we can also design special solutions for your specific requirements. All nanoruler samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.
The following image shows GATTA-STED 3D 90R Expert Line nanorulers on an Abberior Instruments MINFIELD STED.
Image courtesy by Abberior Instruments GmbH.
More information can be found in the following publication:
Göttfert, F. et al. Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc Natl Acad Sci USA (2017). doi:10.1073/pnas.1621495114
Nanoruler | STED 3D 90R |
---|---|
Color | red |
Available fluorophores | ATTO 647N |
Recommended laser source | 630 - 650 nm |
Surface density | ≈ 1/µm² |
Axial distance | 90 nm |
Level | Expert |
Price | 400,-€ |
Expert: Samples which demand perfectly aligned microscopes and very experienced users which are able to optimize acquisition and data evaluation.
Fluorophore | Our notation |
Excitation / nm |
Emission / nm |
ATTO 647N | R | 647 | 664 |