Nanorulers from the GATTA-PAINT HiRes series serve all advantages of the classical GATTA-PAINT nanorulers. Additionally, they include special DNA-PAINT based fiducial markers for a precise and reliable drift correction. These fiducial markers outclass common fiducial markers based on fluorescent beads by having a stable connection to the surface and emitting a signal which does neither bleach nor exceed the detector’s dynamic range.
For the GATTA-PAINT HiRes nanorulers we offer the mark-to-mark distances in the sizes 20 nm, 40 nm and 80 nm. Each size is available in the colors red (ATTO 655) or green (ATTO 542).
GATTA-PAINT HiRes 80R | GATTA-PAINT HiRes 80G | GATTA-PAINT HiRes 80G | |
---|---|---|---|
Color | red | yellow | green |
Available fluorophores | ATTO 655 | Cy3B | ATTO 542 |
Recommended laser source | 625 - 665 nm | 560 - 590 nm | 530 - 560 nm |
Surface density Fiducial Marker | ≈ 0,1/µm² | ≈ 0,1/µm² | ≈ 0,1/µm² |
Surface density PAINT Nanoruler | ≈ 1/μm² | ≈ 1/μm² | ≈ 1/μm² |
PAINT Nanoruler Distance | 2x 80 nm | 2x 80 nm | 2x 80 nm |
Level | Beginner | Beginner | Beginner |
Price | 400,-€ | 400,-€ | 400,-€ |
GATTA-PAINT HiRes 40R | GATTA-PAINT HiRes 40Y | GATTA-PAINT HiRes 40G | |
---|---|---|---|
Color | red | yellow | green |
Available fluorophores | ATTO 655 | Cy3B | ATTO 542 |
Recommended laser source | 625 - 665 nm | 560 - 590 nm | 530 - 560 nm |
Surface density Fiducial Marker | ≈ 0,1/µm² | ≈ 0,1/µm² | ≈ 0,1/µm² |
Surface density PAINT Nanoruler | ≈ 1/μm² | ≈ 1/μm² | ≈ 1/μm² |
PAINT Nanoruler Distance | 2x 40 nm | 2x 40 nm | 2x 40 nm |
Level | Advanced | Advanced | Advanced |
Price | 400,-€ | 400,-€ | 400,-€ |
GATTA-PAINT HiRes 20R | GATTA-PAINT HiRes 20Y | GATTA-PAINT HiRes 20G | |
---|---|---|---|
Color | red | yellow | green |
Available fluorophores | ATTO 655 | Cy3B | ATTO 542 |
Recommended laser source | 625 - 665 nm | 560 - 590 nm | 530 - 560 nm |
Surface density Fiducial Marker | ≈ 0,1/µm² | ≈ 0,1/µm² | ≈ 0,1/µm² |
Surface density PAINT Nanoruler | ≈ 1/μm² | ≈ 1/μm² | ≈ 1/μm² |
PAINT Nanoruler Distance | 2x 20 nm | 2x 20 nm | 2x 20 nm |
Level | Expert | Expert | Expert |
Price | 400,-€ | 400,-€ | 400,-€ |
Beginner: Samples to check the basic functions of your microscope. Easy to resolve, also for beginners in the field of super-resolution microscopy.
Advanced: Samples to optimize the settings of your microscope. Advanced experience is necessary in order to resolve the nanorulers.
Expert: Samples which demand perfectly aligned microscopes and very experienced users which are able to optimize acquisition and data evaluation.
Fluorophore | Our notation |
Excitation / nm |
Emission / nm |
ATTO 655 | R | 655 | 680 |
ATTO 542 | G | 542 | 562 |
The following video describes the basic functions of our GATTAnalysis software using GATTA-PAINT HiRes Nanoruler with fiducial markers.