GATTA-PAINT Nanoruler

GATTA-PAINT Nanoruler GATTA-PAINT Nanoruler

Nanorulers from the GATTA-PAINT series are the ideal resolution rulers for any type of localization based super-resolution microscopy. Due to the mechanism for providing the blink kinetics which is based on the DNA PAINT technology the samples are nearly free of photo bleaching. In addition the design containing three emitting points allows the acquisition of very eye-catching images.

We offer for the GATTA-PAINT nanorulers the mark-to-mark distances in the sizes 40 nm and 80 nm. Both sizes are available in the colors red (ATTO 655), green (ATTO 542) or yellow (Cy3B) as well as in the color combination red/green (ATTO 655 & ATTO 542) or red/yellow (ATTO 655 & ATTO Cy3B).

product properties of GATTA-PAINT 80 nm Nanoruler

Nanoruler PAINT 80R PAINT 80Y PAINT 80G
Color red yellow green
Available fluorophores ATTO 655 Cy3B ATTO 542
Recommended laser source 625 - 665 nm 560 – 590 nm 530 - 560 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Distance 2x 80 nm 2x 80 nm 2x 80 nm
Level Beginner Beginner Beginner
Price 350,-€ 350,-€ 350,-€

product properties of GATTA-PAINT 40 nm Nanoruler

Nanoruler PAINT 40R PAINT 40Y PAINT 40G
Color red yellow green
Available fluorophores ATTO 655 Cy3B ATTO 542
Recommended laser source 625 - 665 nm 560 – 590 nm 530 - 560 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Distance 2x 40 nm 2x 40 nm 2x 40 nm
Level Advanced Advanced Advanced
Price 350,-€ 350,-€ 350,-€

product properties of GATTA-PAINT 80 nm and 40 nm Multicolor Nanoruler

Nanoruler PAINT 80RG PAINT 80RY PAINT 40RG PAINT 40RY
Color red / green red / yellow red / green red / yellow
Available fluorophores ATTO 655 / ATTO 542 ATTO 655 / Cy3B ATTO 655 / ATTO 542 ATTO 655 / Cy3B
Recommended laser source 625 – 665 nm / 530 – 560 nm 625 – 665 nm / 560 – 590 nm 625 – 665 nm / 530 – 560 nm 625 – 665 nm / 560 – 590 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Distance 2x 80 nm 2x 80 nm 2x 40 nm 2x 40 nm
Level Advanced Advanced Advanced Advanced
Price 400,-€ 400,-€ 400,-€ 400,-€

New super-resolution Button GATTA-PAINT Nanoruler for higher temperature measuremnts

DNA-PAINT measurements are typically performed on microscopy platforms at room temperature (21–25 °C). However, certain advanced systems (e.g., ONI Nanoimager, ONI Aplo Scope, among others) are equipped with temperature-controlled stages that operate at elevated temperatures (29–31 °C). These conditions significantly influence DNA-PAINT kinetics, accelerating the binding and unbinding rates of imager strands and thereby compromising the detection of reliable localization events.

To address this challenge, we offer specialized GATTA-PAINT HEAT nanorulers, engineered for temperature-optimized performance. These standards enable robust and reproducible measurements under elevated stage temperatures, ensuring consistent DNA-PAINT imaging quality across a wider range of experimental conditions.

product properties of GATTA-PAINT HEAT 80 nm and 40 nm Nanoruler

Nanoruler PAINT 80R HEAT PAINT 80Y HEAT PAINT 40R HEAT PAINT 40Y HEAT
Color red yellow red yellow
Available fluorophores ATTO 655 Cy3B ATTO 655 Cy3B
Recommended laser source 625 – 665 nm 560 – 590 nm 625 – 665 nm 560 – 590 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Distance 2x 80 nm 2x 80 nm 2x 40 nm 2x 40 nm
Level Beginner Beginner Beginner Beginner
Price 350,-€ 350,-€ 350,-€ 350,-€

product properties of GATTA-PAINT HEAT 80 nm and 40 nm Multicolor Nanoruler

Nanoruler PAINT 80RY HEAT PAINT 40RY HEAT
Color red / yellow red / yellow
Available fluorophores ATTO 655 / Cy3B ATTO 655 / Cy3B
Recommended laser source 625 – 665 nm / 560 – 590 nm 625 – 665 nm / 560 – 590 nm
Surface density ≈ 1/µm² ≈ 1/µm²
Distance 2x 80 nm 2x 40 nm
Level Advanced Advanced
Price 400,-€ 400,-€

Beginner: Samples to check the basic functions of your microscope. Easy to resolve, also for beginners in the field of microscopy.

Advanced: Samples to optimize the settings of your microscope. Advanced experience is necessary in order to resolve the nanorulers.

fluorophore properties

Fluorophore Our notation
 Excitation / nm
 Emission / nm
ATTO 655 R 655 680
Cy3B Y 560 571
ATTO 542 G 542 562
Product sheets

Download iconDownload GATTA-PAINT product sheet

Nanoruler product-sheetInstruction sheet - How to use Nanoruler

Full size super-resolution Images

Download iconsuper-resolution Image of GATTA-PAINT 80R after 2 months at 20°C

Download iconFull size super-resolution Image of GATTA-PAINT 80R

Nanoruler product-sheetFull size super-resolution Image of GATTA-PAINT 80G

Nanoruler product-sheetFull size super-resolution Image of Custom-PAINT 80B

Nanoruler product-sheetFull size super-resolution Image of GATTA-PAINT 80RG

Nanoruler product-sheetFull size super-resolution Image of GATTA-PAINT 40RG

Nanoruler product-sheetFull size super-resolution Image of 9months old GATTA-PAINT 80R

Nanoruler product-sheetFull size super-resolution Image of 12months old GATTA-PAINT 80R

Raw Data (.zip File)

Nanoruler product-sheetComplete Raw Data of GATTA-PAINT 80R   (ca. 430MB)

References / Experience Reports

Leica Microsystems Oxford NanoImaging

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